), which is the same angular place as being the covalent connections among the ChVLig OB area along with the strands in the latch. Consequently, Nature has located very diverse structural solutions to the issue of DNA envelopment by ATP-dependent ligases.

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). The NTase domain binds to your damaged and intact DNA strands in the key groove flanking the nick in addition to within the slight groove over the three′-OH side on the nick (supplemental Fig. S3). The OB area binds through the minor groove within the deal with of your duplex at the rear of the nick.

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